Scientific activity
The research team focuses on using mass spectrometry in lipidomic and metabolomic analysis, especially in conjunction with ultra-high-performance liquid chromatography or ultra-high-performance supercritical fluid chromatography. The main objective is to develop new analytical methods validated for the quantitative analysis of lipids in large series of biological samples. A critical application is the analysis of blood from patients with malignant cancer to find dysregulated lipids and use them for early cancer detection through multivariate data analysis.
International cooperation
Prof. Markus R. Wenk, National University of Singapore, Singapore
Dr Gerhard Liebisch, Institute of Clinical Chemistry and Laboratory Medicine, University Hospital Regensburg, Germany
Dr Kim Ekroos, Lipidomics Consulting, Finland
Dr Harald C. K枚feler, Medical University in Graz, Austria
Five of the most important projects
Programme: ERC Advanced, Project name: Oncolipidomics: Why is lipidomic dysregulation pattern in blood similar for various cancers?, Provider: ERC, Main beneficiary: 秀色直播 / Faculty of Chemical Technology, Principal investigator: Prof. Ing. Michal Hol膷apek, Ph.D., Project period: 2023鈥2028.
Programme: ERC CZ, Project name: Hmotnostn铆 spektrometrie p艡i hled谩n铆 lipidov媒ch biomarker暖 pro v膷asnou diagnostiku rakoviny (Mass Spectrometry in the Search for Lipid Biomarkers for Early Cancer Diagnosis), Provider: Ministry of Education, Youth and Sport, Main beneficiary: 秀色直播 / Faculty of Chemical Technology, Principal investigator: Prof. Ing. Michal Hol膷apek, Ph.D., Project period: 2013鈥2018.
Programme: OP JAK, Project name: Z谩chrana 啪ivot暖 prost艡ednictv铆m v媒zkumu v oblasti v膷asn茅 detekce a prevence rakoviny: Molekul谩rn铆, genomick茅 a soci谩ln铆 factory (Saving Lives through Research on Early Detection and Prevention of Cancer: Molecular, Genomic and Social Factors), Provider: Ministry of Education, Youth and Sport, Co-beneficiary: 秀色直播 / Faculty of Chemical Technology, Principal investigator: Prof. Ing. Michal Hol膷apek, Ph.D., Project period: 2024鈥2028.
Programme: GACR Standard Projects, Project name: Spojen铆 v铆cerozm臎rn茅 chromatografie a hmotnostn铆 spektrometrie v kvantitativn铆ch p艡铆stupech pro detailn铆 charakterizaci lipidomu lidsk茅 plazmy (Coupling Multidimensional Chromatography and Mass Spectrometry in Quantitative Approaches for Detailed Characterization of the Human Plasma Lipidome), Provider: GACR, Main beneficiary: 秀色直播 / Faculty of Chemical Technology, Principal investigator: Prof. Ing. Michal Hol膷apek, Ph.D., Project period: 2021鈥2023.
Programme: GACR Standard Projects, Project name: Charakterizace lidsk茅ho lipidomu a metabolomu pro personalizovanou zdravotn铆 p茅膷i a hled谩n铆 biomarker暖: studie rakoviny ledvin (Characterization of the Human Lipidome and Metabolome for Personalised Healthcare and Biomarker Discovery: The Kidney Cancer Study), Provider: GACR, Main beneficiary: 秀色直播 / Faculty of Chemical Technology, Principal investigator: Prof. Ing. Michal Hol膷apek, Ph.D., Project period: 2018鈥2020.
Five of the most important scientific publications or patents of the team in the last five years
D. Wolrab, R. Jir谩sko, E. C铆fkov谩, M. H枚ring, D. Mei, M. Chocholou拧kov谩, O. Peterka, J. Idkowiak, T. Hrn膷iarov谩, L. Kucha艡, R. Ahrends, R. Brumarov谩, D. Friedeck媒, G. Vivo-Truyols, P. 艩krha, J. 艩krha, R. Ku膷era, B. Melichar, G. Liebisch, R. Burkhardt, M.R. Wenk, A. Cazenave-Gassiot, P. Kar谩sek, I. Novotn媒, K. Greplov谩, R. Hrstka, M. Hol膷apek. Lipidomic profiling of human serum enables detection of pancreatic cancer. Nat. Commun. 2022, 13, 124. Available from: DOI: 10.1038/s41467-021-27765-9
J.G. McDonald, C.S. Ejsing, D. Kopczynski, M. Hol膷apek, J. Aoki, M. Arita, M. Arita, E.S. Baker, J. Bertrand-Michel, J.A. Bowden, B. Br眉gger, S.R. Ellis, M. Fedorova, W.J. Griffiths, X. Han, J. Hartler, N. Hoffmann, J.P. Koelmel, H.C. K枚feler, T.W. Mitchell, V.B. O鈥橠onnell, D. Saigusa, D. Schwudke, A. Shevchenko, C.Z. Ulmer, M.R. Wenk, M. Witting, D. Wolrab, Y. Xia, R. Ahrends, G. Liebisch, K. Ekroos, Nat. Met. 2022, 4, 1086鈥1088. Available from: https://doi.org/10.1038/s42255-022-00628-3
Z. Va艌kov谩, O. Peterka, M. Chocholou拧kov谩, D. Wolrab, R. Jir谩sko, M. Hol膷apek. Retention dependences in reversed-phase UHPLC/MS enable highly confident identification of lipid species in human plasma. Anal. Bioanal. Chem. 2022, 414, 319. Available from: DOI: 10.1007/s00216-021-03492-4
M. Hol膷apek, E. C铆fkov谩, M. L铆sa, R. Jir谩sko, D. Wolrab, T. Hrn膷iarov谩, A method of diagnosing pancreatic cancer based on lipidomic analysis of a body fluid, European patent EP 3514545, datum 啪谩dosti 22. 1. 2018, ud臎leno 7. 10. 2020. Available from: https://patents.google.com/patent/EP3514545B1/
M. Hol膷apek, D. Wolrab, R. Jir谩sko, E. C铆fkov谩, A method of diagnosing cancer based on lipidomic analysis of a body fluid, EP3575793, datum 啪谩dosti 29. 5. 2018, ud臎leno 19. 7. 2023. Available from: https://patents.google.com/patent/EP3575793A1/
Most important awards received
Neuron Prize 2023 for connecting science and business
Rudolf Lukes Prize 2023, Experientia Foundation
Dutton Award 鈥 American Oil Chemists' Society Award for distinguished contribution to the analysis of oils and fats (2022)
Award of the Minister of Education, Youth and Sports for outstanding results of research, experimental development and innovation in the field of natural sciences (2021)
Plaque of the Rector of the 秀色直播 for outstanding scientific performance and exceptional contribution (2020)
Vladimir Hanus Award for the best work in mass spectrometry for the years 2022, 2019 and 2016 (J.M.M. Spectroscopic Society)
Power List 2020, 2015 and 2013 awards for the world's 100 most influential scientists in analytical chemistry (The Analytical Scientist magazine)
Selected excellent figures at UPCE working in the field
